Composite

Part:BBa_K2477008

Designed by: Paula Kleyman, Thea Ornstein, Yuzhu Shi, Joyce Song   Group: iGEM17_UMaryland   (2017-10-26)


GFP based fusaric acid biosensor

This part includes constitutive production of the phlF repressor. The repressive ability of this regulator is stabilized by fusaric acid and salicylate. The PhlF protein inhibits the immediately downstream promoter which regulates the transcription of GFP. A decrease in GFP production should therefore accompanies the presence of fusaric acid/salicylate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1527


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Categories
Parameters
None